pZ_P-GAP-LacI-Mit1AD
(Plasmid
#126722)
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PurposeLacI-Mit1AD on pGAPZ_ A expression vector
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 126722 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepGAPZ_ A
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 3147
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Vector typeYeast Expression
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Selectable markersZeocin
Growth in Bacteria
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Bacterial Resistance(s)Bleocin (Zeocin), 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namehybrid lacI-Mit1 activation domain coding sequence
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Alt namelacI-mit1AD
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SpeciesEscherichia coli, Komagataella phaffii
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Insert Size (bp)3456
- Promoter GAP
Cloning Information
- Cloning method Gibson Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pZ_P-GAP-LacI-Mit1AD was a gift from Menghao Cai (Addgene plasmid # 126722 ; http://n2t.net/addgene:126722 ; RRID:Addgene_126722) -
For your References section:
Engineered ethanol-driven biosynthetic system for improving production of acetyl-CoA derived drugs in Crabtree-negative yeast. Liu Y, Bai C, Liu Q, Xu Q, Qian Z, Peng Q, Yu J, Xu M, Zhou X, Zhang Y, Cai M. Metab Eng. 2019 Jul;54:275-284. doi: 10.1016/j.ymben.2019.05.001. Epub 2019 May 9. 10.1016/j.ymben.2019.05.001 PubMed 31077813