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PurposeFluorescent reporter for cyclin E/A-CDK and CDK4/6 activities
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 126679 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepLenti
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Vector typeMammalian Expression, Lentiviral
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Selectable markersBlasticidin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameRb (a.a 886-928)
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SpeciesH. sapiens (human)
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Tag
/ Fusion Protein
- mCherry
Resource Information
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Supplemental Documents
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLenti-DHB-mVenus-p2a-mCherry-CDK4KTR was a gift from Hee Won Yang (Addgene plasmid # 126679 ; http://n2t.net/addgene:126679 ; RRID:Addgene_126679) -
For your References section:
Stress-mediated exit to quiescence restricted by increasing persistence in CDK4/6 activation. Yang HW, Cappell SD, Jaimovich A, Liu C, Chung M, Daigh LH, Pack LR, Fan Y, Regot S, Covert M, Meyer T. Elife. 2020 Apr 7;9. pii: 44571. doi: 10.7554/eLife.44571. 10.7554/eLife.44571 PubMed 32255427