GFP-rab9 DN
(Plasmid #12664)

• Depositing Lab: Richard Pagano
• Publication: Choudhury et al J Clin Invest. 2002 Jun . 109(12):1541-50.

Backbone

• Vector backbone: p-EGFP-C1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4700
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: RAB9 (Homo sapiens)
• Alt name: GFP-rab9 dominant negative
• Species: H. sapiens (human)
• Insert Size (bp): 678
• Mutation: changed serine 21 to asparagine
• GenBank ID: NM_004251
• Entrez Gene: RAB9A (a.k.a. RAB9)
• Tags / Fusion Proteins:
  • GFP (N terminal on backbone)
  • HA (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BAMHI (not destroyed)
• 3′ cloning site: BAMHI (not destroyed)
• 5′ sequencing primer: EGFP-C

Resource Information

• A portion of this plasmid was derived from a plasmid made by: IMAGE Clone collection at ATCC
• Articles Citing this Plasmid:
  • 5 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry