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Addgene

pLGB31
(Plasmid #126621)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 126621 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    new backbone
  • Modifications to backbone
    NEBuilder assembly reaction to fuse: a) ss-bfe1 gene and MCS, amplified from pLGB13 b) rhamnose-inducible promoter and RhaR transcription factor, amplified from pLGB30 c) vector backbone (oriT/V, bla gene, inulin selection cassette) amplified from pLGB29
  • Vector type
    allellic exchange in bacteria
  • Selectable markers
    inulin (bacteroidales)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    S17-lambda pir
  • Copy number
    Low Copy

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLGB31 was a gift from Laurie Comstock (Addgene plasmid # 126621 ; http://n2t.net/addgene:126621 ; RRID:Addgene_126621)

  • For your References section:

    Streamlined Genetic Manipulation of Diverse Bacteroides and Parabacteroides Isolates from the Human Gut Microbiota. Garcia-Bayona L, Comstock LE. mBio. 2019 Aug 13;10(4). pii: mBio.01762-19. doi: 10.1128/mBio.01762-19. 10.1128/mBio.01762-19 PubMed 31409684
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