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Purpose(Empty Backbone) Suicide vector for allelic replacement in Bacteroides, inulin selection and aTC-inducible ss-Bfe1 counterselection
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 126619 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonenew backbone
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Vector typeallellic exchange in bacteria
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Selectable markersinulin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)S17-lambda pir
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Copy numberLow Copy
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLGB29 was a gift from Laurie Comstock (Addgene plasmid # 126619 ; http://n2t.net/addgene:126619 ; RRID:Addgene_126619) -
For your References section:
Streamlined Genetic Manipulation of Diverse Bacteroides and Parabacteroides Isolates from the Human Gut Microbiota. Garcia-Bayona L, Comstock LE. mBio. 2019 Aug 13;10(4). pii: mBio.01762-19. doi: 10.1128/mBio.01762-19. 10.1128/mBio.01762-19 PubMed 31409684
