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Addgene

pLGB28
(Plasmid #126617)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 126617 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pNBU2
  • Modifications to backbone
    NEBuilder assembly reaction to fuse: a) pNBU2 backbone removing ermG cassette b) PBT1311 promoter and RBS, amplified from pMM553 (Mimee et al., 2015)(this promoter will drive expression of the inulin cassette) c) BACOVA_04504 and BACOVA_04505 from B. ovatus ATCC 8483 d) BACOVA_04501 from B. ovatus ATCC 8483
  • Vector type
    Unspecified ; chromosomal integration suicide vector
  • Selectable markers
    inulin (Bacteroidales)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    S17-lambda pir
  • Copy number
    Low Copy

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLGB28 was a gift from Laurie Comstock (Addgene plasmid # 126617 ; http://n2t.net/addgene:126617 ; RRID:Addgene_126617)

  • For your References section:

    Streamlined Genetic Manipulation of Diverse Bacteroides and Parabacteroides Isolates from the Human Gut Microbiota. Garcia-Bayona L, Comstock LE. mBio. 2019 Aug 13;10(4). pii: mBio.01762-19. doi: 10.1128/mBio.01762-19. 10.1128/mBio.01762-19 PubMed 31409684
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