pB-bsd-Flag-CNP
(Plasmid #126608)

• Purpose: Expresses N-terminal Flag-tagged human CNP
• Depositing Lab: Xiaozhong Wang
• Publication: Unlu et al J Biol Chem. 2018 Dec 14;293(50):19365-19376. doi: 10.1074/jbc.RA118.004872. Epub 2018 Oct 24.

Backbone

• Vector backbone: PB-bsd
• Vector type: Mammalian Expression
• Selectable markers: Blasticidin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: 2'3' cyclic nucleotide phosphodiesterase
• Alt name: CNP
• Species: M. musculus (mouse)
• Insert Size (bp): 1200
• Entrez Gene: CNP (a.k.a. CNP1, HLD20)
• Promoter: CAG
• Tag / Fusion Protein:
  • Flag (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: GAGCCTCTGCTAACCATGTTCATGCC

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • piggyBac Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Construct contains a non-canonical FLAG tag of DYKDDDDR rather than the typical DYKDDDDK that is functional in the experience of the depositor.