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Addgene

pLQ-KO-tgt50
(Plasmid #126578)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 126578 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    ColE1 origin, AmpR, Rep(+), cat resistance
  • Backbone size w/o insert (bp) 4000
  • Vector type
    Bacterial Expression, CRISPR
  • Selectable markers
    Chloramphenicol in S. aureus.

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    High copy in E. coli, low copy in S. aureus. chloramphenicol resistance in S. aureus
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Cas9-Pxyltet-sgRNA-Pspac-donor-tgt50
  • Species
    S. pyogenes
  • Insert Size (bp)
    8000

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Addgene NGS identified a D24E and C-terminal truncation of Cas9. The depositing lab has confirmed that these should not affect function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLQ-KO-tgt50 was a gift from Sheng Yang (Addgene plasmid # 126578 ; http://n2t.net/addgene:126578 ; RRID:Addgene_126578)

  • For your References section:

    CRISPR/Cas9-based efficient genome editing in Staphylococcus aureus. Liu Q, Jiang Y, Shao L, Yang P, Sun B, Yang S, Chen D. Acta Biochim Biophys Sin (Shanghai). 2017 Sep 1;49(9):764-770. doi: 10.1093/abbs/gmx074. 10.1093/abbs/gmx074 PubMed 28910979
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