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Addgene

XF1 GAL Smad3
(Plasmid #12635)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 12635 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    XF1 Gal
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Smad3
  • Alt name
    MADR3
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1275
  • Entrez Gene
    SMAD3 (a.k.a. HSPC193, HsT17436, JV15-2, LDS1C, LDS3, MADH3, hMAD-3, hSMAD3, mad3)
  • Tag / Fusion Protein
    • Gal4 DBD (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer Gal4 forward
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The Gal4 DNA Binding Domain (aa1-147) was fused to full-length Smad3. See author's map for details.

Also see: Feng et al (1998) Genes and Development 12, 2153-2163.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    XF1 GAL Smad3 was a gift from Rik Derynck (Addgene plasmid # 12635 ; http://n2t.net/addgene:12635 ; RRID:Addgene_12635)

  • For your References section:

    Smad3 and Smad4 cooperate with c-Jun/c-Fos to mediate TGF-beta-induced transcription. Zhang Y, Feng XH, Derynck R. Nature. 1998 Aug 27. 394(6696):909-13. 10.1038/29814 PubMed 9732876
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