p1148-pCSDest-AcrIIA4-mCherry
(Plasmid
#126204)
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PurposeMammalian expression of AcrIIA4 fused to mCherry
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 126204 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCSDest2
- Backbone size w/o insert (bp) 4896
- Total vector size (bp) 5157
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namecodon-optimized AcrIIA4
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Insert Size (bp)261
- Promoter CMV
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Tags
/ Fusion Proteins
- FLAG/NLS (C terminal on insert)
- mCherry (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer ATTTAGGTGACACTATAGA
- 3′ sequencing primer TAATACGACTCACTATAG (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
p1148-pCSDest-AcrIIA4-mCherry was a gift from Erik Sontheimer (Addgene plasmid # 126204 ; http://n2t.net/addgene:126204 ; RRID:Addgene_126204) -
For your References section:
Tissue-restricted Genome Editing in vivo Specified by MicroRNA-repressible Anti-CRISPR Proteins. Lee J, Mou H, Ibraheim R, Liang SQ, Liu P, Xue W, Sontheimer EJ. RNA. 2019 Aug 22. pii: rna.071704.119. doi: 10.1261/rna.071704.119. 10.1261/rna.071704.119 PubMed 31439808
Map uploaded by the depositor.
