pLaNGo11-3
(Plasmid #126054)

• Purpose: A self-assembling split-GFP based Golden Gate vector to determine protein targeting to plastids in plant cell
• Depositing Lab: Ralf-Bernd Klösgen
• Publication: Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs.230839. doi: 10.1242/jcs.230839.

Backbone

• Vector backbone: pAGM4723
• Total vector size (bp): 11613
• Vector type: Plant Expression ; Binary

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol and Kanamycin, 25 & 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): ccdB Survival
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: chloroplastic FNR transit peptide
• Alt name: FNR (tp)
• Species: Spinacia oleracea
• Insert Size (bp): 834
• GenBank ID: M86349.1
• Tag / Fusion Protein:
  • GFP1-10 (C terminal on insert)

Gene/Insert 2

• Gene/Insert name: ccdB cassette (BsaI)
• Species: E. coli
• Insert Size (bp): 1458

Gene/Insert 3

• Gene/Insert name: GFP11x3 (5 aa linker)
• Alt name: GFP11 M3
• Insert Size (bp): 201

Resource Information

• Supplemental Documents:
  • pLaNGo11-3.gbk
• A portion of this plasmid was derived from a plasmid made by: Huang Lab via Addgene

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please incubate bacteria at 30 degrees Celsius after transformations to increase efficiency. Further growth after transformants are obtained should be performed at 37 degrees Celsius.