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Addgene

pSELt_S7.A
(Plasmid #125835)

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Item Catalog # Description Quantity Price (USD)
Plasmid 125835 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    Custom Backbone
  • Vector type
    Bacterial Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    ACP-2xAmb-pheS
  • Alt name
    acyl carrier protein fused to pheS with two amber stop codons in the linker sequence
  • Species
    E. coli
  • Insert Size (bp)
    1239
  • GenBank ID
    WP_001637778.1 EGT68318.1
  • Promoter pr.trpL*

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer CACTTCGCAGAATAAATAAATCCTGGTGTCC
  • 3′ sequencing primer GCGTTAAATTTTTGTTAAATCAGCTC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSELt_S7.A was a gift from Andrew Ellington (Addgene plasmid # 125835 ; http://n2t.net/addgene:125835 ; RRID:Addgene_125835)

  • For your References section:

    Evolving Orthogonal Suppressor tRNAs To Incorporate Modified Amino Acids. Maranhao AC, Ellington AD. ACS Synth Biol. 2017 Jan 20;6(1):108-119. doi: 10.1021/acssynbio.6b00145. Epub 2016 Sep 21. 10.1021/acssynbio.6b00145 PubMed 27600875
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