pET-EIN-AlkBH5(66-292)
(Plasmid #125732)

• Purpose: Plasmid with EIN as a solubility and expression enhancing tag to increase production of hard to express proteins such as AlkBH5 and TEV cleaving site for the removal of the fusion tag.
• Depositing Lab: Vincenzo Venditti
• Publication: Khatiwada et al Protein Expr Purif. 2019 Nov 15;167:105540. doi: 10.1016/j.pep.2019.105540.

Backbone

• Vector backbone: pET21a
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 5443
• Total vector size (bp): 6906
• Modifications to backbone: Added EIN fusion Tag and AlkBH5(66-292) gene
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: AlkBH5(66-292)
• Species: H. sapiens (human)
• Insert Size (bp): 684
• GenBank ID: 54890
• Entrez Gene: ALKBH5 (a.k.a. ABH5, OFOXD, OFOXD1)
• Promoter: T7
• Tag / Fusion Protein:
  • 6xHis and EIN (N terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: T7 Fwd
• 3′ sequencing primer: pET Rev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry