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Addgene

Ndel1 RNAi
(Plasmid #12573)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 12573 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSilencer 2.0-U6
  • Backbone manufacturer
    Ambion
  • Backbone size w/o insert (bp) 3130
  • Vector type
    Mammalian Expression, RNAi

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Ndel1 shRNA
  • Alt name
    Ndel1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    40
  • Entrez Gene
    NDEL1 (a.k.a. EOPA, MITAP1, NDE1L1, NDE2, NUDEL)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Ndel1 base pair 276-294: GCAGG TCTCA GTGTT AGAA.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Ndel1 RNAi was a gift from Li-Huei Tsai (Addgene plasmid # 12573 ; http://n2t.net/addgene:12573 ; RRID:Addgene_12573)

  • For your References section:

    Ndel1 operates in a common pathway with LIS1 and cytoplasmic dynein to regulate cortical neuronal positioning. Shu T, Ayala R, Nguyen MD, Xie Z, Gleeson JG, Tsai LH. Neuron. 2004 Oct 14. 44(2):263-77. 10.1016/j.neuron.2004.09.030 PubMed 15473966
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