pPAICS-2XStrep
(Plasmid
#125683)
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Purposeexpresses human PAICS protein with a C-terminal 2XStrep affinity tag
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 125683 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCI-neo
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Backbone manufacturerClontech
- Backbone size w/o insert (bp) 5472
- Total vector size (bp) 6828
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Modifications to backboneaddition of 2X Strep-tag II by restriction cloning at the end of the MCS
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namePAICS
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Alt namephosphoribosylaminoimidazole carboxylase and phosphoribosylaminoimidazolesuccinocarboxamide synthase
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SpeciesH. sapiens (human)
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Insert Size (bp)1275
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Entrez GenePAICS (a.k.a. ADE2, ADE2H1, AIRC, PAICSD, PAIS)
- Promoter CMV
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Tag
/ Fusion Protein
- 2X Strep-Tag II (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NheI (not destroyed)
- 3′ cloning site EcoRI (destroyed during cloning)
- 5′ sequencing primer GAC ATC CAC TTT GCC TTT CT (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pPAICS-2XStrep was a gift from Stephen Benkovic (Addgene plasmid # 125683 ; http://n2t.net/addgene:125683 ; RRID:Addgene_125683) -
For your References section:
Mapping Post-Translational Modifications of de Novo Purine Biosynthetic Enzymes: Implications for Pathway Regulation. Liu C, Knudsen GM, Pedley AM, He J, Johnson JL, Yaron TM, Cantley LC, Benkovic SJ. J Proteome Res. 2019 May 3;18(5):2078-2087. doi: 10.1021/acs.jproteome.8b00969. Epub 2019 Apr 18. 10.1021/acs.jproteome.8b00969 PubMed 30964683