PCD FL4X
(Plasmid #12565)

• Depositing Lab: Carl Novina
• Publication: Wang et al Mol Cell. 2006 May 19. 22(4):553-60.

Backbone

• Vector backbone: pcDNA3.1
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 7200
• Vector type: Mammalian Expression, Luciferase ; Reporter for translation repression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Four CXCR4 imperfect binding sites
• Alt name: CXCR4
• Species: H. sapiens (human)
• Insert Size (bp): 100
• Entrez Gene: CXCR4 (a.k.a. CD184, D2S201E, FB22, HM89, HSY3RR, LAP-3, LAP3, LCR1, LESTR, NPY3R, NPYR, NPYRL, NPYY3R, WHIM, WHIMS, WHIMS1)
• Tag / Fusion Protein:
  • luciferase (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XbaI (not destroyed)
• 3′ cloning site: ApaI (not destroyed)
• 5′ sequencing primer: n/a
• 3′ sequencing primer: BGH rev primer

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Firefly luciferase was cloned into the HindIII/SpeI sites of pcDNA3.1. Four imperfect complementary binding sites to the antisense strand of CXCR4 siRNA were cloned in the XbaI/ApaI sites in the 3'UTR of luciferase.

Author's sequence data was obtained by using a primer reading forward from inside the luciferase gene.

Imperfect binding sequence is - 5'-AAGTTTTCACAAAGCTAACA