pPPAT-2XStrep
(Plasmid #125620)

• Purpose: expresses human PPAT protein with a C-terminal 2XStrep affinity tag
• Depositing Lab: Stephen Benkovic
• Publication: Liu et al J Proteome Res. 2019 May 3;18(5):2078-2087. doi: 10.1021/acs.jproteome.8b00969. Epub 2019 Apr 18.

Backbone

• Vector backbone: pCI-neo
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 5472
• Total vector size (bp): 7095
• Modifications to backbone: addition of 2X Strep-tag II by restriction cloning at the end of the MCS
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: PPAT
• Alt name: phosphoribosyl pyrophosphate amidotransferase
• Species: H. sapiens (human)
• Insert Size (bp): 1551
• GenBank ID: NM_002703
• Entrez Gene: PPAT (a.k.a. ATASE, GPAT, PRAT)
• Promoter: CMV
• Tag / Fusion Protein:
  • 2X Strep-Tag II (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: KpnI (not destroyed)
• 5′ sequencing primer: GAC ATC CAC TTT GCC TTT CT

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry