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Addgene

pFD152
(Plasmid #125546)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 125546 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pFD116 with an optimized RBS to use in E. coli
  • Backbone size w/o insert (bp) 3729
  • Total vector size (bp) 9131
  • Vector type
    CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    optimized RBS
  • Insert Size (bp)
    44
  • Promoter anhydrotetracycline Ptet promoter

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer CTTTTTCCGTGATGGTAACTAACTAATAACGTAACGTGACTGGC
  • 3′ sequencing primer GTTACGTTATTAGTTAGTTACCATCACGGAAAAAGGTTATGCTG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFD152 was a gift from David Bikard (Addgene plasmid # 125546 ; http://n2t.net/addgene:125546 ; RRID:Addgene_125546)

  • For your References section:

    Gene silencing with CRISPRi in bacteria and optimization of dCas9 expression levels. Depardieu F, Bikard D. Methods. 2019 Aug 1. pii: S1046-2023(18)30497-3. doi: 10.1016/j.ymeth.2019.07.024. 10.1016/j.ymeth.2019.07.024 PubMed 31377338
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