pBabe N-Ras 61K
(Plasmid #12543)

• Depositing Lab: Channing Der
• Publication: Khosravi-Far et al Mol Cell Biol. 1996 Jul . 16(7):3923-33.

Backbone

• Vector backbone: pBabe-Puro
• Backbone size w/o insert (bp): 5169
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: NRas 61K
• Alt name: NRAS
• Species: H. sapiens (human)
• Insert Size (bp): 570
• Mutation: 61K constitutively activated.
• Entrez Gene: NRAS (a.k.a. ALPS4, CMNS, KRAS, N-ras, NCMS, NRAS1, NS6)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: pBabe 5'
• 3′ sequencing primer: pBABE-3

Resource Information

• Articles Citing this Plasmid:
  • 17 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The original N-Ras was cloned as a full-length cDNA (containing 5' and 3' UTRs) Bam-Bam into pCGN, and was therefore transfered via Bam cuts into other vectors, including pBABE, and orientation checked. So, a 1.2 kb band is expected. There should be about 100 bp of 5'UTR, 527 bp N-Ras, and the rest 3'UTR