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pFPV25.1_rpsM_mCerulean3
(Plasmid #124904)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 124904 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pFPV25.1
  • Modifications to backbone
    GFPmut3 was taken out and was replaced with mCerulean
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    rpsM promoter and mCerulean3
  • Species
    S. typhimurium (promoter seq), mCerulean3 codon-optimized for S. typhimurium

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFPV25.1_rpsM_mCerulean3 was a gift from Markus Covert (Addgene plasmid # 124904 ; http://n2t.net/addgene:124904 ; RRID:Addgene_124904)

  • For your References section:

    Escalating Threat Levels of Bacterial Infection Can Be Discriminated by Distinct MAPK and NF-kappaB Signaling Dynamics in Single Host Cells. Lane K, Andres-Terre M, Kudo T, Monack DM, Covert MW. Cell Syst. 2019 Mar 27;8(3):183-196.e4. doi: 10.1016/j.cels.2019.02.008. Epub 2019 Mar 20. 10.1016/j.cels.2019.02.008 PubMed 30904375
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