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Addgene

pCold CL7-Cas9
(Plasmid #124890)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 124890 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCold I
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Cas9

Cloning Information

  • Cloning method Ligation Independent Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCold CL7-Cas9 was a gift from Yi Liu (Addgene plasmid # 124890 ; http://n2t.net/addgene:124890 ; RRID:Addgene_124890)

  • For your References section:

    Co-expression of Cas9 and single-guided RNAs in Escherichia coli streamlines production of Cas9 ribonucleoproteins. Qiao J, Li W, Lin S, Sun W, Ma L, Liu Y. Commun Biol. 2019 May 3;2:161. doi: 10.1038/s42003-019-0402-x. eCollection 2019. 10.1038/s42003-019-0402-x PubMed 31069270
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