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Purposeobtaining full Cas9 RNP
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 124890 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCold I
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCas9
Cloning Information
- Cloning method Ligation Independent Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCold CL7-Cas9 was a gift from Yi Liu (Addgene plasmid # 124890 ; http://n2t.net/addgene:124890 ; RRID:Addgene_124890) -
For your References section:
Co-expression of Cas9 and single-guided RNAs in Escherichia coli streamlines production of Cas9 ribonucleoproteins. Qiao J, Li W, Lin S, Sun W, Ma L, Liu Y. Commun Biol. 2019 May 3;2:161. doi: 10.1038/s42003-019-0402-x. eCollection 2019. 10.1038/s42003-019-0402-x PubMed 31069270