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Addgene

Human SHIP2
(Plasmid #124645)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 124645 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3 HIS C
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5514
  • Total vector size (bp) 9978
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    INPPL1
  • Alt name
    SHIP2; 51C protein
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    4503
  • Mutation
    coding sequence starts at amino acid 18 as calculated from the 1258 amino acids full length sequence in Pesesse et al Biochem Biophys Res Commun. 1997 Oct 29;239(3):697-700 "Identification of a second SH2-domain-containing protein closely related to the phosphatidylinositol polyphosphate 5-phosphatase SHIP".
  • GenBank ID
    NM_001567
  • Entrez Gene
    INPPL1 (a.k.a. OPSMD, SHIP2)
  • Tag / Fusion Protein
    • His Xpress (N-terminal) (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T3
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Human SHIP2 was a gift from Christophe Erneux (Addgene plasmid # 124645 ; http://n2t.net/addgene:124645 ; RRID:Addgene_124645)

  • For your References section:

    The Src homology 2 domain containing inositol 5-phosphatase SHIP2 is recruited to the epidermal growth factor (EGF) receptor and dephosphorylates phosphatidylinositol 3,4,5-trisphosphate in EGF-stimulated COS-7 cells. Pesesse X, Dewaste V, De Smedt F, Laffargue M, Giuriato S, Moreau C, Payrastre B, Erneux C. J Biol Chem. 2001 Jul 27;276(30):28348-55. doi: 10.1074/jbc.M103537200. Epub 2001 May 10. 10.1074/jbc.M103537200 PubMed 11349134
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