SpyDock
(Plasmid #124618)

• Purpose: Expresses SpyDock to bind SpyTag non-covalently for affinity purification
• Depositing Lab: Mark Howarth
• Publication: Khairil Anuar et al Nat Commun. 2019 Apr 15;10(1):1734. doi: 10.1038/s41467-019-09678-w.

Backbone

• Vector backbone: pDEST14
• Backbone manufacturer: Thermo Fisher Scientific
• Backbone size w/o insert (bp): 3641
• Total vector size (bp): 4034
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SpyDock
• Species: Synthetic
• Insert Size (bp): 393
• Mutation: E77A mutation prevents isopeptide bond formation, S49C mutation enables attachment to solid support. Mutations A89P, Q97D, K108E helps better SpyTag binding.
• Promoter: T7
• Tag / Fusion Protein:
  • His6 (N terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: T7F
• 3′ sequencing primer: T7R

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry