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Addgene

pET28C-tCorn
(Plasmid #124588)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 124588 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET28c
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    tRNA-Corn
  • Promoter lacI

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EagI or NotI (not destroyed)
  • 3′ cloning site SacII (overlaps with an EagI/NotI site, so cleave 3' before 5') (not destroyed)
  • 5′ sequencing primer -
  • 3′ sequencing primer -
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28C-tCorn was a gift from Samie Jaffrey (Addgene plasmid # 124588 ; http://n2t.net/addgene:124588 ; RRID:Addgene_124588)

  • For your References section:

    Imaging RNA polymerase III transcription using a photostable RNA-fluorophore complex. Song W, Filonov GS, Kim H, Hirsch M, Li X, Moon JD, Jaffrey SR. Nat Chem Biol. 2017 Nov;13(11):1187-1194. doi: 10.1038/nchembio.2477. Epub 2017 Sep 25. 10.1038/nchembio.2477 PubMed 28945233
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