pLenti_Puro_DEST mouse_p65promoter_mouse_p65-Clover
(Plasmid
#124549)
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PurposeLentiviral vector to express p65-Clover under p65 promoter (With puromycin resistance)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 124549 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepLenti _Puro_DEST
- Backbone size w/o insert (bp) 7475
- Total vector size (bp) 11302
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Vector typeMammalian Expression, Lentiviral
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert namep65 promoter- p65 CDS-Clover
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SpeciesM. musculus (mouse)
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Insert Size (bp)3827
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Tag
/ Fusion Protein
- Clover (C terminal on insert)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLenti_Puro_DEST mouse_p65promoter_mouse_p65-Clover was a gift from Markus Covert (Addgene plasmid # 124549 ; http://n2t.net/addgene:124549 ; RRID:Addgene_124549) -
For your References section:
Measuring Signaling and RNA-Seq in the Same Cell Links Gene Expression to Dynamic Patterns of NF-kappaB Activation. Lane K, Van Valen D, DeFelice MM, Macklin DN, Kudo T, Jaimovich A, Carr A, Meyer T, Pe'er D, Boutet SC, Covert MW. Cell Syst. 2017 Apr 26;4(4):458-469.e5. doi: 10.1016/j.cels.2017.03.010. Epub 2017 Apr 5. 10.1016/j.cels.2017.03.010 PubMed 28396000