pGlomyc-Frat1
(Plasmid #124499)

• Purpose: Expresses myc-tagged mouse FRAT1 in mammalian cells (N-terminal tag)
• Depositing Lab: Renee van Amerongen
• Publication: van der Wal et al PLoS One. 2020 Jan 21;15(1):e0227435. doi: 10.1371/journal.pone.0227435. eCollection 2020.

Backbone

• Vector backbone: pGlomyc (based on pCDNA3.1)
• Backbone manufacturer: Olivier Destree
• Backbone size w/o insert (bp): 5500
• Total vector size (bp): 6400
• Modifications to backbone: The pGlomyc vector backbone contains 5’ and 3’ globin UTRs and the pCDNA1.1 BamHI-NsiI polylinker in the backbone of pCDNA3.1(+).
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Frat1
• Species: M. musculus (mouse)
• Insert Size (bp): 860
• GenBank ID: NM_008043.3
• Entrez Gene: Frat1 (a.k.a. AW060382)
• Promoter: CMV
• Tag / Fusion Protein:
  • myc tag (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (destroyed during cloning)
• 3′ cloning site: EcorI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: BGH polyA

Resource Information

• Supplemental Documents:
  • Cloning history of the construct
• A portion of this plasmid was derived from a plasmid made by: Construct was originally cloned in the lab of prof.dr. Anton Berns, Netherlands Cancer Institute.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

References:

https://www.ncbi.nlm.nih.gov/pubmed/?term=10534617

https://www.ncbi.nlm.nih.gov/pubmed/?term=15073180

https://www.ncbi.nlm.nih.gov/pubmed/?term=19802005 Please visit https://www.biorxiv.org/content/10.1101/512426v1 for BioRxiv preprint