pAAV-CAG-mNeonGreen
(Plasmid #124101)

• Purpose: Expression of the fluorescent protein mNeonGreen in mammalian cells
• Depositing Lab: Pantelis Tsoulfas
• Publication: AAV expression plasmids for production of mNeonGreen (unpublished)

Backbone

• Vector backbone: 741
• Backbone manufacturer: Scott Sternson
• Backbone size w/o insert (bp): 4715
• Total vector size (bp): 5456
• Vector type: Mammalian Expression, AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: mNeonGreen
• Species: Synthetic; Brachiostoma lanceolatum
• Insert Size (bp): 741
• Mutation: Codon usage was optimized for Homo Sapiens
• GenBank ID: KC295282.1
• Promoter: CAG

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: aaagagacagcaaccagg
• 3′ sequencing primer: cggcttctggcgtgtgac

Resource Information

• A portion of this plasmid was derived from a plasmid made by: The mNeonGreen codon usage was adapted to Homo Sapiens
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • mNeonGreen Label License
• Industry Terms:
  • Not Available to Industry