pFS177
(Plasmid #12380)

• Depositing Lab: Nick Rhind
• Publication: Sivakumar et al Methods. 2004 Jul . 33(3):213-9.

Backbone

• Vector backbone: pART1
• Backbone size w/o insert (bp): 7300
• Vector type: Yeast Expression
• Selectable markers: LEU2

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: human equilibrating nucleoside transporter 1
• Alt name: hENT1
• Species: H. sapiens (human)
• Insert Size (bp): 1400
• Mutation: N81S and M84V
• GenBank ID: AAC51103
• Entrez Gene: SLC29A1 (a.k.a. AUG, ENT1, hENT1)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (destroyed during cloning)
• 3′ cloning site: SacI (not destroyed)
• 5′ sequencing primer: n/a
• 3′ sequencing primer: n/a

Resource Information

• A portion of this plasmid was derived from a plasmid made by: IMAGE Consortium

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that Addgene's sequencing results identified N81S and M84V mutations in the hENT1 sequence when compared to GenBank entry AAC51103. These mutations were not intentional and this plasmid is exactly as described in the associated article listed below. The depositing laboratory states that the hENT1 in the plasmid is functional.