pcDNA3-Lyn-AktAR
(Plasmid #123356)

• Purpose: FRET-based biosensor for monitoring Akt activity in lipid raft plasma membrane regions.
• Depositing Lab: Jin Zhang
• Publication: Gao et al Mol Biol Cell. 2008 Oct;19(10):4366-73. doi: 10.1091/mbc.E08-05-0449. Epub 2008 Aug 13.

Backbone

• Vector backbone: pcDNA3
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5415
• Total vector size (bp): 7461
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Lyn-AktAR
• Species: S. cerevisiae (budding yeast), Synthetic
• Insert Size (bp): 2046
• Promoter: CMV
• Tags / Fusion Proteins:
  • Cerulean (N terminal on insert)
  • cpVenus[E172] (C terminal on insert)
  • N-terminal targeting sequence from Lyn kinase (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: CMV forward
• 3′ sequencing primer: BGH reverse

Resource Information

• Supplemental Documents:
  • pcDNA3-Lyn-AktAR.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry