pSB167 - pL2_pSB90_2x35S::fLUC-I::tNOS_tMAS::rLUC-I::pMAS
(Plasmid #123200)

• Purpose: binary plant vector for transient expression of a Renilla luciferase (rLUC, with intron) and a firefly luciferase (fLUC, with intron) in plants
• Depositing Labs: Erin Cram, Carolyn Lee-Parsons
• Publication: Mortensen et al Front Plant Sci. 2019 Jun 11;10:755. doi: 10.3389/fpls.2019.00755. eCollection 2019.

Backbone

• Vector backbone: pSB90
• Backbone manufacturer: Modified from pAGM4723 (Weber et al., 2011; DOI:10.1371/journal.pone.0016765) by the addition of the VirGN54D gene
• Backbone size w/o insert (bp): 5851
• Vector type: Plant Expression, Luciferase

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: 2x35S::fLUC-I::tNOS tMAS::rLUC-I::pMAS
• Species: Synthetic
• Insert Size (bp): 4698

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Unknown (unknown if destroyed)
• 3′ cloning site: Unknown (unknown if destroyed)
• 5′ sequencing primer: n/a

Resource Information

• Supplemental Documents:
  • pSB167 - pL2_pSB90_2x35S__fLUC-I__tNOS_tMAS__rLUC-I__pMAS.gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
  • Renilla Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Vector for transient expression of a Renilla luciferase and a firefly luciferase (rLUC and fLUC, with introns for avoiding expression in Agrobacteria) in plants. Increased virulence in Agrob. through VirGN54D in the vector backbone. Can be used for dual-luciferase assays