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pLenti-CAG-NOTCH2NL-delta EGF repeats (N2NL-delta EGF)-ires-EGFP
(Plasmid #122955)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 122955 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    lentiviral
  • Backbone manufacturer
    Dr. Cecile Charrier
  • Vector type
    Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Growth instructions
    Stbl3 is also recommended.
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    NOTCH2NL-delta EGF repeats
  • Alt name
    N2NL-delta EGF
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    177
  • Mutation
    deletion of EGF repeats from full length NOTCH2NLB
  • Entrez Gene
    NOTCH2NLB (a.k.a. N2N, NOTCH2NL, NOTCH2NLA)
  • Promoter CAG promoter
  • Tag / Fusion Protein
    • 6xhis tag (C terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLenti-CAG-NOTCH2NL-delta EGF repeats (N2NL-delta EGF)-ires-EGFP was a gift from Pierre Vanderhaeghen (Addgene plasmid # 122955 ; http://n2t.net/addgene:122955 ; RRID:Addgene_122955)

  • For your References section:

    Human-Specific NOTCH2NL Genes Expand Cortical Neurogenesis through Delta/Notch Regulation. Suzuki IK, Gacquer D, Van Heurck R, Kumar D, Wojno M, Bilheu A, Herpoel A, Lambert N, Cheron J, Polleux F, Detours V, Vanderhaeghen P. Cell. 2018 May 31;173(6):1370-1384.e16. doi: 10.1016/j.cell.2018.03.067. Epub 2018 May 31. 10.1016/j.cell.2018.03.067 PubMed 29856955
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