pMIG-w
(Plasmid #12282)

• Purpose: (Empty Backbone) a bicistronic IRES-GFP containing vector which is a variant of pMIG that contains a posttranscriptional regulatory element from the woodchuck hepatitis virus
• Depositing Lab: Luk Parijs
• Publication: Refaeli et al J Exp Med. 2002 Oct 7. 196(7):999-1005.

Backbone

• Vector backbone: modified pMIG
• Backbone size (bp): 7166
• Modifications to backbone: modification of pMIG vector to contain WRE element.
• Vector type: Mammalian Expression, Retroviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Growth instructions: Use stbl3 cells from Invitrogen to decrease chance of recombination.
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: None
• Tag / Fusion Protein:
  • IRES GFP (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: pLXSN 5'

Resource Information

• Supplemental Documents:
  • pMIG-w EcoRI/SalI digest
• Articles Citing this Plasmid:
  • 8 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please acknowledge Dr. Yosef Refaeli (National Jewish Medical and Research Center) when using this plasmid in future publications.

pMIGw is a bicistronic IRES-GFP containing vector which is a variant of pMIG that contains a posttranscriptional regulatory element from the woodchuck hepatitis virus. The WRE element increases export to the cytoplasm and boosts protein expression by 2-10 fold.

IMPORTANT: This plasmid grows extremely slowly! Addgene recommends that when prepping DNA, you let your culture grow 18-20 hours (or more). Overnight will probably not be sufficient for a good prep.