pBabe puro H-Ras G12V T35S
(Plasmid #12274)

• Depositing Lab: Bob Weinberg
• Publication: Rangarajan et al Cancer Cell. 2004 Aug . 6(2):171-83.

Backbone

• Vector backbone: pBABE-puro
• Backbone size w/o insert (bp): 5150
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: H-Ras G12V T35S
• Alt name: HRas
• Species: H. sapiens (human)
• Mutation: Contains G12V activating mutation. The T35S mutation allows binding and activation of Raf, but not of PI3K or Ral-GEF.
• Entrez Gene: HRAS (a.k.a. C-BAS/HAS, C-H-RAS, C-HA-RAS1, CTLO, H-RASIDX, HAMSV, HRAS1, RASH1, p21ras)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: SalI (not destroyed)
• 5′ sequencing primer: pBABE 5'
• 3′ sequencing primer: pBABE 3'

Resource Information

• A portion of this plasmid was derived from a plasmid made by: See Rodriguez-Viciana, P., et al. (1997) Cell 89, pp. 457
• Articles Citing this Plasmid:
  • 9 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

A BamHI-SalI fragment from the pWZL Blast ras mutants were individually cloned into the pBABE puro vector directionally.