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Addgene

P22_wtCP_SP141his
(Plasmid #122651)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 122651 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRSF-Duet
  • Backbone manufacturer
    Novagen
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Use bacterial strain BL21(DE3) for expression.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    P22 Coat: WT / SP: 6xHis-SP141
  • Species
    bacteriophage P22
  • Mutation
    truncated SP, P120H in the capsid scaffold (see depositor comments below)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer n/a
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Depositor confirms that P120H mutation does not affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    P22_wtCP_SP141his was a gift from Trevor Douglas (Addgene plasmid # 122651 ; http://n2t.net/addgene:122651 ; RRID:Addgene_122651)
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