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pLenti-hPGK-eGFP-SynaptoZip
(Plasmid #122523)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 122523 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLenti
  • Backbone manufacturer
    L Naldini
  • Backbone size w/o insert (bp) 7085
  • Total vector size (bp) 9809
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    eGFP-SynaptoZip
  • Alt name
    GreenZip
  • Alt name
    GZ
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    1278
  • GenBank ID
    MF797884
  • Entrez Gene
    Vamp2 (a.k.a. RATVAMPB, RATVAMPIR, SYB, Syb2)
  • Promoter hPGK
  • Tags / Fusion Proteins
    • eGFP (N terminal on insert)
    • Myc (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AgeI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer GCAGCACGACTTCTTCAAGTC
  • 3′ sequencing primer ATTGGTTAAATCCAAGGGAAGG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLenti-hPGK-eGFP-SynaptoZip was a gift from Antonio Malgaroli (Addgene plasmid # 122523 ; http://n2t.net/addgene:122523 ; RRID:Addgene_122523)

  • For your References section:

    Functional mapping of brain synapses by the enriching activity-marker SynaptoZip. Ferro M, Lamanna J, Ripamonti M, Racchetti G, Arena A, Spadini S, Montesano G, Cortese R, Zimarino V, Malgaroli A. Nat Commun. 2017 Oct 31;8(1):1229. doi: 10.1038/s41467-017-01335-4. 10.1038/s41467-017-01335-4 PubMed 29089485
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