pHR-HP1α-miRFP670-Cry2WT
(Plasmid #122442)

• Purpose: Expresses disordered protein HP1α fused with fluorescent protein miRFP670 and optogenetic protein Cry2WT
• Depositing Lab: Clifford Brangwynne
• Publication: Shin et al Cell. 2018 Nov 29;175(6):1481-1491.e13. doi: 10.1016/j.cell.2018.10.057.

Backbone

• Vector backbone: pHR
• Backbone size w/o insert (bp): 9000
• Total vector size (bp): 12142
• Vector type: Mammalian Expression, Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: HP1 alpha
• Species: H. sapiens (human)
• Insert Size (bp): 570
• Entrez Gene: CBX5 (a.k.a. HEL25, HP1, HP1A, HP1alpha)
• Promoter: SFFV
• Tags / Fusion Proteins:
  • miRFP670 (C terminal on insert)
  • Cry2WT (C terminal on insert)

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: n/a
• 3′ sequencing primer: n/a

Resource Information

• A portion of this plasmid was derived from a plasmid made by: HP1 alpha was subcloned from Addgene 17652 (Cheutin et al., Science, 2003). miRFP670 was from Addgene plasmid #79987 (Shcherbakova et al., Nat. Commun., 2016).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Our Cry2WT contains an additional 29 amino acids at the C-terminus of Cry2PHR the same as our previously deposited plasmid Addgene #101223 (Shin et al., Cell, 2017).