pHR-pSFFV-dCas9-SunTag-P2A-BFP-dWPRE
(Plasmid #122151)

• Purpose: Expresses dCas9-SunTag(24X) fusion and a fluorescent indicator BFP.
• Depositing Lab: Clifford Brangwynne
• Publication: Shin et al Cell. 2018 Nov 29;175(6):1481-1491.e13. doi: 10.1016/j.cell.2018.10.057.

Backbone

• Vector backbone: pHR
• Backbone size w/o insert (bp): 9000
• Total vector size (bp): 15861
• Vector type: Mammalian Expression, Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: dCas9 fused to SunTag(24x)
• Insert Size (bp): 6909
• Promoter: SFFV
• Tags / Fusion Proteins:
  • NLS (N terminal on insert)
  • 2xNLS-P2A-BFP (C terminal on insert)

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: n/a
• 3′ sequencing primer: n/a

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Addgene Plasmid #60910. (Tanenbaum et al., Cell, 2014)
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid is the same as Addgene_60910 except for the dSV40 promoter was replaced with SFFV promoter. Each of the 24 copies of GCN4_v4 peptide can be recognized by scFv-GCN4.