pEBG-Pak1 83-149 L107F
(Plasmid #12215)

• Depositing Lab: Jonathan Chernoff
• Publication: Xiao et al J Biol Chem. 2002 Jan 11. 277(2):883-6.

Backbone

• Vector backbone: pEBG
• Backbone size w/o insert (bp): 6100
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Pak1
• Alt name: Pak1 AID L107F
• Species: H. sapiens (human)
• Insert Size (bp): 200
• Mutation: Autoinhibitory domain (aa 83-149) with L107F mutation, rendering inhibitor inactive
• Entrez Gene: PAK1 (a.k.a. IDDMSSD, PAKalpha, alpha-PAK, p65-PAK)
• Tag / Fusion Protein:
  • GST (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: Acc65I (not destroyed)
• 5′ sequencing primer: pGEX 5'

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry