pIVT
(Plasmid
#122139)
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Purpose(Empty Backbone) For making cRNA appropriate for microinjection
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 122139 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepIVT
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Backbone manufacturerCarmen Williams
- Backbone size (bp) 3059
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Modifications to backboneMCS: SphI, Pst1, Sal1, Xba1, BamHI, SmaI, Acc651, KpnI, SacI
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Vector typeFor in vitro transcription
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pIVT was a gift from Carmen Williams (Addgene plasmid # 122139 ; http://n2t.net/addgene:122139 ; RRID:Addgene_122139) -
For your References section:
Alterations of PLCbeta1 in mouse eggs change calcium oscillatory behavior following fertilization. Igarashi H, Knott JG, Schultz RM, Williams CJ. Dev Biol. 2007 Dec 1;312(1):321-30. Epub 2007 Sep 26. 10.1016/j.ydbio.2007.09.028 PubMed 17961538
Map uploaded by the depositor.
