-
PurposeExpress a scFv which binds to GCN4, sfGFP and VP64 cassette from the PGK promoter
-
Depositing Lab
-
Sequence Information
-
Sequences (1) — Accept Affinity Reagent Sequence Policy
-
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 122133 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepHR
-
Vector typeMammalian Expression, Lentiviral, CRISPR
-
Selectable markerssfGFP
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)NEB Stable
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert namescFv GCN4-sfGFP-VP64
- Promoter PGK
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer Unknown (Common Sequencing Primers)
Resource Information
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pHR-PGK-scFv GCN4-sfGFP-VP64 was a gift from Stanley Qi (Addgene plasmid # 122133 ; http://n2t.net/addgene:122133 ; RRID:Addgene_122133) -
For your References section:
CRISPR Activation Screens Systematically Identify Factors that Drive Neuronal Fate and Reprogramming. Liu Y, Yu C, Daley TP, Wang F, Cao WS, Bhate S, Lin X, Still C 2nd, Liu H, Zhao D, Wang H, Xie XS, Ding S, Wong WH, Wernig M, Qi LS. Cell Stem Cell. 2018 Sep 28. pii: S1934-5909(18)30440-5. doi: 10.1016/j.stem.2018.09.003. 10.1016/j.stem.2018.09.003 PubMed 30318302