pCMV6M-PAK1 T423E
(Plasmid #12208)

• Depositing Lab: Jonathan Chernoff
• Publication: Sells et al Curr Biol. 1997 Mar 1. 7(3):202-10.

Backbone

• Vector backbone: pCMV6
• Backbone size w/o insert (bp): 4900
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Pak1
• Species: H. sapiens (human)
• Insert Size (bp): 2000
• Mutation: Activated Pak1: T423E
• Entrez Gene: PAK1 (a.k.a. IDDMSSD, PAKalpha, alpha-PAK, p65-PAK)
• Tag / Fusion Protein:
  • Myc (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SalI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: TCGTTTAGTGAACCGTCAG
• 3′ sequencing primer: GGAACTTCCAAGGCCAGGA

Resource Information

• Articles Citing this Plasmid:
  • 16 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

There is also an additional mutation in this plasmid - L516I. The L516I mutation, or SNP, has been present from the very beginning (~1995). The crystal structure of Pak1 uses this clone, so it is present there too. The depositor does not think it affects function in any way.

Pak1 may contain a G401S mutation. Depositor states that this mutation should not affect plasmid function.