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Addgene

GBSGFP insulator
(Plasmid #121975)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 121975 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pJC4-5
  • Modifications to backbone
    chick insulator - 8xdel5 hsp68 promoter -EGFP-polyA- chick insulator
  • Vector type
    mouse transgenesis

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GBS(long)-GFP-insulator
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Gnb5 (a.k.a. GBS, Gbeta5, Hg2e, flr)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (destroyed during cloning)
  • 3′ cloning site BamHI (destroyed during cloning)
  • 5′ sequencing primer unknown
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GBSGFP insulator was a gift from James Briscoe (Addgene plasmid # 121975 ; http://n2t.net/addgene:121975 ; RRID:Addgene_121975)

  • For your References section:

    Gene regulatory logic for reading the Sonic Hedgehog signaling gradient in the vertebrate neural tube. Balaskas N, Ribeiro A, Panovska J, Dessaud E, Sasai N, Page KM, Briscoe J, Ribes V. Cell. 2012 Jan 20;148(1-2):273-84. doi: 10.1016/j.cell.2011.10.047. 10.1016/j.cell.2011.10.047 PubMed 22265416
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