pHR-BRD4ΔN-mCherry-sspB
(Plasmid #121968)

• Purpose: Expresses fusion of disordered protein BRD4(462-1362), fluorescent protein mCherry, and sspB which upon light activation binds to iLID.
• Depositing Lab: Clifford Brangwynne
• Publication: Shin et al Cell. 2018 Nov 29;175(6):1481-1491.e13. doi: 10.1016/j.cell.2018.10.057.

Backbone

• Vector backbone: pHR
• Backbone size w/o insert (bp): 9000
• Total vector size (bp): 12827
• Vector type: Mammalian Expression, Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: BRD4
• Species: H. sapiens (human)
• Insert Size (bp): 2703
• Mutation: Deleted amino acids 1-461
• Entrez Gene: BRD4 (a.k.a. CAP, CDLS6, FSHRG4, HUNK1, HUNKI, MCAP)
• Promoter: SFFV
• Tag / Fusion Protein:
  • mCherry-sspB (C terminal on insert)

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: n/a
• 3′ sequencing primer: n/a

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Vector contains SspB, a bacterial protein which binds exposed SsrA tag (from iLID) at high affinity.