pCS2 Geminin
(Plasmid
#12174)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 12174 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCS2+
- Backbone size w/o insert (bp) 4095
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGeminin
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SpeciesX. laevis (frog)
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Insert Size (bp)2600
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GenBank IDNP_056979
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Entrez Genegmnn (a.k.a. gem, geminin, MGC83313)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer SP6
- 3′ sequencing primer T3 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCS2 Geminin was a gift from Marc Kirschner (Addgene plasmid # 12174 ; http://n2t.net/addgene:12174 ; RRID:Addgene_12174) -
For your References section:
Geminin, an inhibitor of DNA replication, is degraded during mitosis. McGarry TJ, Kirschner MW. Cell. 1998 Jun 12. 93(6):1043-53. 10.1016/S0092-8674(00)81209-X PubMed 9635433