pMB84 (Nf2 C-term)
(Plasmid
#12169)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 12169 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCR-Blunt II-TOPO
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 3500
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Vector typeMammalian Expression, Cre/Lox
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNf2 C-terminal half
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Alt nameNf2
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SpeciesM. musculus (mouse)
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Insert Size (bp)836
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MutationC-terminal half of Nf2 cDNA
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Entrez GeneNf2 (a.k.a. merlin)
Cloning Information
- Cloning method TOPO Cloning
- 5′ sequencing primer n/a (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
In situ probe for Nf2.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMB84 (Nf2 C-term) was a gift from Tyler Jacks (Addgene plasmid # 12169 ; http://n2t.net/addgene:12169 ; RRID:Addgene_12169) -
For your References section:
The Nf2 tumor suppressor regulates cell-cell adhesion during tissue fusion. McLaughlin ME, Kruger GM, Slocum KL, Crowley D, Michaud NA, Huang J, Magendantz M, Jacks T. Proc Natl Acad Sci U S A. 2007 Feb 27. 104(9):3261-6. 10.1073/pnas.0700044104 PubMed 17360635