pRG/III-hs-MBP-T43NTR-TrxA-H10
(Plasmid #121677)

• Purpose: Bacterial expression of neurotensin receptor fusion protein
• Depositing Labs: Reinhard Grisshammer, Richard Henderson
• Publication: Grisshammer et al Protein Expr Purif. 1997 Oct;11(1):53-60. doi: 10.1006/prep.1997.0766.

Backbone

• Vector backbone: pRG/III-hs-MBP
• Backbone size w/o insert (bp): 6991
• Total vector size (bp): 8498
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: neurotensin receptor
• Alt name: NTSR1
• Species: R. norvegicus (rat)
• Insert Size (bp): 1161
• Mutation: deleted amino acids 1-42
• Entrez Gene: Ntsr1 (a.k.a. Ntsr)
• Promoter: lac
• Tag / Fusion Protein:
  • TrxA-H10 (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: MalE2
• 3′ sequencing primer: M13-For

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Internal plasmid code # 624