FU_tetO_dCas9-VP192-T2A-EGFP
(Plasmid
#121173)
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PurposeLentiviral construct for inducible expression of dCas9-VP192 transcriptional activator and EGFP.
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 121173 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneFU_tetOp
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Vector typeMammalian Expression, Lentiviral, CRISPR ; Inducible by doxycycline
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Selectable markersEGFP
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namedCas9-VP192-T2A-EGFP
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SpeciesStreptococcus pyogenes
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Tag
/ Fusion Protein
- T2A-EGFP-ires-puro
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made bydCas9-VP192 cloned from pCXLE-dCas9VP192-T2A-EGFP (Plasmid #69536).
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
This lentivirus needs to be used together with a doxycycline responsive transcriptional activator expressing lentivirus like FUdeltaGW-rtTA (Plasmid #19780) and lentivirus expressing guide RNAs targeting a promoter for activation (e.g. lentiGuide-Puro
(Plasmid #52963)).
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
FU_tetO_dCas9-VP192-T2A-EGFP was a gift from Timo Otonkoski (Addgene plasmid # 121173 ; http://n2t.net/addgene:121173 ; RRID:Addgene_121173)
