pLV-shEsr2
(Plasmid #120722)

• Purpose: Lentiviral vector that expresses GFP and an shRNA targeting Esr2 (in pLL3.7).
• Depositing Lab: Amy Lasek
• Publication: Vandegrift et al J Neurosci. 2020 Jul 1;40(27):5196-5207. doi: 10.1523/JNEUROSCI.2364-19.2020. Epub 2020 Jun 1.

Backbone

• Vector backbone: pLL3.7
• Backbone manufacturer: MIT
• Backbone size w/o insert (bp): 7649
• Total vector size (bp): 7700
• Vector type: Mammalian Expression, Lentiviral, RNAi

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Esr2
• gRNA/shRNA sequence: gtacgaagacagagaagtg
• Species: M. musculus (mouse)
• Entrez Gene: Esr2 (a.k.a. ER[b], ERbeta, Estrb)
• Promoter: mouse U6
• Tag / Fusion Protein:
  • GFP

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HpaI (destroyed during cloning)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: U6

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry