ARF17 pBluescript
(Plasmid #12068)

• Depositing Lab: David Bartel
• Publication: Mallory et al Plant Cell. 2005 May . 17(5):1360-75.

Backbone

• Vector backbone: pBluescript II SK+
• Backbone manufacturer: Stratagene
• Backbone size w/o insert (bp): 3000
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: ARF17
• Alt name: auxin response transcription factor 17
• Species: A. thaliana (mustard weed)
• Insert Size (bp): 6600
• Entrez Gene: ARF17 (a.k.a. AT1G77850, F28K19.6, F28K19_6, auxin response factor 17)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: SpeI (not destroyed)
• 5′ sequencing primer: T7

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The genomic sequence of ARF17 (At1g77850), including 1.9 and 1.7 kb of putative 5' and 3' regulatory sequences, respectively, was cloned as an 6.6-kb EcoRI-SpeI fragment into pBluescript II SK+ (Stratagene, La Jolla, CA) from the BAC F28K19. See "sequence" for full insert sequence.