ScFv-2ERT2-V
(Plasmid #120553)

• Purpose: 3rd gen transfer vector. Encode an antibody that binds to the GCN4 peptide from the SunTag system, and is fused to sfGFP, 2 tandem ERT2 and VP64.
• Depositing Lab: Yu Wang
• Publication: Lu et al Nucleic Acids Res. 2018 Mar 16;46(5):e25. doi: 10.1093/nar/gkx1222.

Backbone

• Vector backbone: pRRL.sin-18.ppy.
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: scFvGCN4, sfGFP, GB1, ERT2, VP64
• Species: Synthetic
• Promoter: hPGK

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Unknown (unknown if destroyed)
• 3′ cloning site: Unknown (unknown if destroyed)
• 5′ sequencing primer: hPGK-F

Resource Information

• A portion of this plasmid was derived from a plasmid made by: ERT2 was cloned from the pAd-CreER plasmid (a gift from T. C. He’s lab, Chicago University); VP64 was cloned from Addgene plasmid #35388; scFvGCN4, sfGFP and GB1 were synthesized according to the sequences reported by Tanenbaum et. Al.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that some discrepancies were found between Addgene's quality control result and the depositor's Genbank sequence. The depositor noted that these discrepancies do NOT affect plasmid function.